Search results for " DNA synthesis"

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Purification of a fetal bovine serum factor that inhibits DNA synthesis in chick embryo retinas

1992

Chick embryo retina DNA synthesis Fetal bovine serum Growth-inhibitory factor In vitro effect Purification procedure
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A factor derived from chick embryo retina which inhibits DNA synthesis of retina itself.

1992

Chick embryo retinas contain a peptide factor that inhibits DNA synthesis in explants of chick embryo retina. The inhibitory factor, obtained by acid/ethanol extraction from 15-day-old chick embryo retinas, was partially purified by affinity chromatography on heparin-sepharose CL-6B and gel filtration on Sephadex G-100. The inhibitor reduced DNA synthesis with maximal effects observed in retinal explants from 7 to 8-day-old chick embryos. The inhibitory effect became apparent after 10 h of incubation and reached the maximum levels after 16 h. DNA-inhibiting activity was heat and acid-stable and was destroyed by trypsin and alkaline treatments. The inhibitory effect was observed in retinal e…

animal structuresChick EmbryoBiologyBiochemistryChromatography AffinityRetinaCellular and Molecular Neurosciencechemistry.chemical_compoundBiological Factorsinhibits DNA synthesisAffinity chromatographyCulture TechniquesmedicineAnimalsRetinaDNA synthesisTissue ExtractsEmbryogenesisEmbryoGeneral MedicineDNATrypsinMolecular biologymedicine.anatomical_structureBiochemistrychemistrySephadexembryonic structuresChromatography GelThymidineCell Divisionmedicine.drugThymidineNeurochemical research
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